Structural analyses of mammalian ribosomal ribonucleic acid and its precursors. Nucleotide sequence of ribosomal 5.8 S ribonucleic acid.

Identification by affinity chromatography of the eukaryotic ribosomal proteins that bind to 5.8 S ribosomal ribonucleic acid.

The proteins that bind to rat liver 5.8 S ribosomal ribonucleic acid were identified by affinity chromatography. The nucleic acid was oxidized with periodate and coupled by its 3'-terminus to Sepharose 4B through and adipic acid dihydrazide spacer. The ribosomal proteins that associate with the immobilized 5.8 S rRNA were identified by polyacrylamide gel electrophoresiss: they were L19, L8, and...

The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.

Ribosomes from the cytoplasm of eukaryotic cells contain two low molecular weight RNA species: the 5 S and the 5.8 S RNA (l-9). Both RNA species are structural components of the large subunit and each is found in equimolar amount to the 28 Si ribosomal RNA (1, 5, 7, 9-11). The 5.8 S RNA is noncovalently bound to the large ribosomal RNA from which it can be dissociated by treatments known to dis...

Nucleotide sequence of rainbow trout (Salmo gairdneri) ribosomal 5.8 S ribonucleic acid.

The nucleotide sequence of rainbow trout (Salmo gairdneri) 5.8 S rRNA was determined to be (formula: see text). The major molecular species is 161 nucleotides long; the sequence differs in only eight nucleotides from that of Man. An estimate of the secondary structure retains essentially all of the features previously described in mammalian 5.8 S RNAs with several interesting variations. The ve...

The binding of transfer ribonucleic acids to 5 S and 5.8 S eukaryotic ribosomal ribonucleic acid-protein complexes.

Rat liver 5 S and 5.8 S rRNAs were oxidized with periodate and the 3' termini were coupled to Sepharose 4B through an adipic acid dihydrazide spacer. Ribosomal proteins were passed through the nucleic acid affinity columns to form ribonucleoprotein complexes containing the nucleic acid and the proteins that bind to it (5 S . L6, L7, L19; and 5.8 S . L6, L19, S9, S13). Pure isoaccepting species ...

The nucleotide sequence of ribosomal 5 S ribonucleic acid from KB cells.